DNA Sequencing (Sanger's)
ICAR-NBFGR has 8-capillary ABI 3500 Genetic Analyzer (Applied Biosystems™). The DNA sequencer is used for sequencing PCR products upto 800 bases read length accurately. It employs BigDye™ Cycle Sequencing Kit, having Fluorescent labelled dideoxy-Nucleoside triphosphates (ddNTPs) for chain termination. For fragment analysis, 5 fluorescent dyes can detect simultaneously for multiplexing in high throughput fragment analysis applications.